RNP Particles, Splicing and Autoimmune Diseases

1 Isolation and Immunochemical Characterization of hnRNP Particles.- 2 The lnRNP Particle -- A Naturally Assembled Complex of Pre-mRNA and Splicing Factors.- 3 Intrinsic Fluorescence Techniques for Studies on Protein-Protein and Protein-RNA Interactions in RNP Particles.- 4 Procedures for Three-Dimensional Reconstruction from Thin Sections with Electron Tomography.- 5 Purification and Electron Microscopy of Spliceosomal snRNPs.- 6 Detection of Autoantibodies to Extractable Cellular Antigens.- 7 Methods in Immunolocalization of Autoantigens.- 8 In vitro Splicing of Pre-mRNA in HeLa Extracts.- Abbreviations.